FORUMRemove chimera
Genevieve asked 1 year ago



Hi, 
I’m now trying to remove chimera sequences on my data. I’m using Vsearch with the default parameters with the Uchime reference data set and I lost almost all of my sequences (ITS2 fungal sequences) from my sample. Its not normal ?  I’m not familiar with all the setting. Can anyone hepl me ?
Thank

1 Answers
Best Answer
Emmanuel answered 1 year ago



Hi Genevieve,
If you send me the parameters you used while running Uchime, I may be able to help you.

Genevieve replied 1 year ago

Hi,

thankl finaly I found the solution

Eloi Mercier replied 1 year ago

Would you be able to post your solution here please? It might help other users with the same problem.

Genevieve replied 1 year ago

Yes of course

I forgot to specify the Select output formats at the end (–nonchimeras).