FORUMRemove chimera
Genevieve asked 10 months ago



  • Hi, 
    I’m now trying to remove chimera sequences on my data. I’m using Vsearch with the default parameters with the Uchime reference data set and I lost almost all of my sequences (ITS2 fungal sequences) from my sample. Its not normal ?  I’m not familiar with all the setting. Can anyone hepl me ?
    Thank

    1 Answers
    Best Answer
    Emmanuel answered 10 months ago



  • Hi Genevieve,
    If you send me the parameters you used while running Uchime, I may be able to help you.

    Genevieve replied 10 months ago

    Hi,

    thankl finaly I found the solution

    Eloi Mercier Staff replied 10 months ago

    Would you be able to post your solution here please? It might help other users with the same problem.

    Genevieve replied 10 months ago

    Yes of course

    I forgot to specify the Select output formats at the end (–nonchimeras).