FORUMCatégorie: BioinformaticsDuplicated Read in RNA-Seq
zhibin personnel demandée il y a 2 ans

Should I remove duplicated reads in RNA-Seq?

1 Réponses
flefebvre personnel répondue il y a 2 ans

Probably not. I would tend to think that without UMIs, single PCR duplicate events are indistinguishable from natural duplication that is likely to occur in RNA-seq.
There are methods like dupRadar to diagnose global duplication problems in samples. I found the Sayol et al. dupradar paper is a particularly interesting read on the topic.